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Risorse bibliografiche
Risorsa bibliografica obbligatoria
Risorsa bibliografica facoltativa
Scheda Riassuntiva
Anno Accademico 2019/2020
Scuola Scuola di Ingegneria Industriale e dell'Informazione
Insegnamento 054345 - BIOPHOTONICS (C.I.)
  • 054344 - OPTICAL MICROSCOPY
Docente Bassi Andrea
Cfu 5.00 Tipo insegnamento Modulo Di Corso Strutturato

Corso di Studi Codice Piano di Studio preventivamente approvato Da (compreso) A (escluso) Insegnamento
Ing Ind - Inf (Mag.)(ord. 270) - MI (486) ENGINEERING PHYSICS - INGEGNERIA FISICA*AZZZZ054850 - OPTICAL MICROSCOPY
054345 - BIOPHOTONICS (C.I.)

Obiettivi dell'insegnamento

Introduce the student to the fundamentals of optical imaging from the micro/nano-scale to the macroscopic level, presenting the physical basis, discussing instrumental implementations and describing examples of applications, with particular emphasis on biophysical and biomedical topics.


Risultati di apprendimento attesi

- Knowledge and understanding

The students will learn the fundamentals of bio-photonics imaging technologies, that operates from the molecular level up to the tissue scale. The student will learn the major applications of optical microscopy in biology and in clinical diagnostics.

- Apply knowledge and understanding

The students will be able to design autonomously an optical system for microscopy and imaging. The students will be able to evaluate the resolution and contrast in optical microscopy and nanoscopy systems.

- Making Judgements

Given a specific optical imaging system, the students will have the ability to analyse the point spread function and to compare the performances of different optical setups.

 - Lifelong learning skills

Students will be capable to understand how to take into account, and how to overcome the diffraction limit in an optical system, with particular emphasis on optical microscopy. Students will be capable to learn autonomously how to use an optical microscope and how to assess its performances.


Argomenti trattati

Optical microscopy and nanoscopy:

  • Point spread function in an optical microscope and 3D imaging theory.
  • Classical microscopy techniques (bright-field, darkfield, phase contrast, fluorescence).
  • Optical sectioning with spatial gating (Confocal microscopy, structured illumination miroscopy, 2-photon microscopy).
  • Tomographic imaging (Filtered back projection algorithm and Optical Projection Tomography).
  • Optical sectioning with temporal gating (Optical Coherent Tomography).
  • Circumventing the resolution limit with optical nanoscopy (Stimulated Emission Depletion and Photo-Activated Localization Microscopy).
  • Applications of microscopy and super-resolution microscopy in biophysics and biomedicine.

Prerequisiti

Knowledge and understanding of the Fourier Transforms.


Modalità di valutazione

The final assessment will be based on a written test with open questions on the topics of the course.


Bibliografia
Risorsa bibliografica obbligatoriaJerome, Introduction to Optical Microscopy, Editore: Cambridge University Press, Anno edizione: 2019, ISBN: 9781108552660

Forme didattiche
Tipo Forma Didattica Ore di attività svolte in aula
(hh:mm)
Ore di studio autonome
(hh:mm)
Lezione
30:00
45:00
Esercitazione
16:00
24:00
Laboratorio Informatico
0:00
0:00
Laboratorio Sperimentale
4:00
6:00
Laboratorio Di Progetto
0:00
0:00
Totale 50:00 75:00

Informazioni in lingua inglese a supporto dell'internazionalizzazione
Insegnamento erogato in lingua Inglese
Disponibilità di materiale didattico/slides in lingua inglese
Disponibilità di libri di testo/bibliografia in lingua inglese
Possibilità di sostenere l'esame in lingua inglese
schedaincarico v. 1.6.5 / 1.6.5
Area Servizi ICT
27/09/2020