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Risorse bibliografiche
Risorsa bibliografica obbligatoria
Risorsa bibliografica facoltativa
Scheda Riassuntiva
Anno Accademico 2019/2020
Scuola Scuola di Ingegneria Industriale e dell'Informazione
Insegnamento 054345 - BIOPHOTONICS (C.I.)
Docente Bassi Andrea , Pifferi Antonio Giovanni
Cfu 10.00 Tipo insegnamento Corso Integrato

Corso di Studi Codice Piano di Studio preventivamente approvato Da (compreso) A (escluso) Insegnamento
Ing Ind - Inf (Mag.)(ord. 270) - MI (486) ENGINEERING PHYSICS - INGEGNERIA FISICA*AZZZZ054849 - DIFFUSE OPTICAL TOMOGRAPHY
054345 - BIOPHOTONICS (C.I.)
054850 - OPTICAL MICROSCOPY

Obiettivi dell'insegnamento

Introduce the student to the fundamentals of optical imaging and tomography at the micro/nano-scale to the macroscopic level, presenting the physical basis, introducing the mathematical tools, discussing instrumental implementations, togetther with photonics advancements, and describing examples of applications, with particular emphasis on biophysical and biomedical topics.

 


Risultati di apprendimento attesi

- Knowledge and understanding

The students will improve knowledge and understanding on wave physics, diffraction and diffusion, light-matter interaction, linear algebra and inverse problems, with specific application to the problem of optical microscopy and optical tomography in diffusive media.

- Apply knowledge and understanding

The students will be able to design autonomously an optical system for microscopy and imaging. The students will be able to evaluate the resolution and contrast in optical microscopy and diffuse tomography systems. The students will be challenged to solve – in the project activity – a problem of tomographic reconstruction implying the application of knowledge on the physics of diffuse optics, modelling of real photonics systems and methods for solving inverse problems.

- Making Judgements

Given a specific optical imaging system, the students will be able to precisely analyse the resolution and contrast.

The students will be required to judge the plausibility of their own project results as well as, in common discussions, of the outcomes of other teams.

- Lifelong learning skills

The students will be capable to understand how to take into account, and how to overcome the effects of diffraction and diffusion in a biological sample.

The students will be faced with the key difference between theoretical models and practical implementation, as well as the ability of problem solving, team-working and workflow organization.


Argomenti trattati

Optical microscopy and nanoscopy:

  • Point spread function in an optical microscope and 3D imaging theory.
  • Classical microscopy techniques (bright-field, darkfield, phase contrast, fluorescence).
  • Optical sectioning with spatial gating (Confocal microscopy, Structured illumination miroscopy, 2-photon microscopy).
  • Tomographic imaging (Filtered back projection algorithm and Optical Projection Tomography).
  • Optical sectioning with temporal gating (Optical Coherence Tomography).
  • Circumventing the resolution limit with optical nanoscopy (Stimulated Emission Depletion and Photo-Activated Localization Microscopy).
  • Applications of microscopy and super-resolution microscopy in biophysics and biomedicine.

Diffuse Optical Tomography:

  • Diffuse optics, time-resolved techniques, frequency-resolved techniques.
  • Applications: optical mammography, cerebral oximetry, functional imaging of brain activity, non-invasive tissue spectroscopy.
  • Photon density waves.
  • Diffuse Optical Tomography in the linear (Born approximation) and non-linear regime.
  • Inverse Problems.
  • Photoacoustics tomography
  • Applications and new perspectives.

Prerequisiti

Basic knowledge on wave physics, linear algebra, diffusion equation for highly scattering media.

Knowledge and understanding of the Fourier Transform.


Modalità di valutazione

The final assessment will be based on a written test with open questions on the topics of the course and of the project activity.


Bibliografia
Risorsa bibliografica obbligatoriaJerome Mertz, Introduction to Optical Microscopy, Editore: Cambridge University Press, Anno edizione: 2019
Risorsa bibliografica obbligatoriaLihong V. Wang, Hsin-I. Wu, Biomedical optics: principles and imaging, Editore: John Wiley & Sons, Anno edizione: 2012

Forme didattiche
Tipo Forma Didattica Ore di attività svolte in aula
(hh:mm)
Ore di studio autonome
(hh:mm)
Lezione
56:00
84:00
Esercitazione
24:00
36:00
Laboratorio Informatico
0:00
0:00
Laboratorio Sperimentale
4:00
6:00
Laboratorio Di Progetto
10:00
30:00
Totale 94:00 156:00

Informazioni in lingua inglese a supporto dell'internazionalizzazione
Insegnamento erogato in lingua Inglese
Disponibilità di materiale didattico/slides in lingua inglese
Disponibilità di libri di testo/bibliografia in lingua inglese
Possibilità di sostenere l'esame in lingua inglese
schedaincarico v. 1.6.1 / 1.6.1
Area Servizi ICT
26/01/2020