This course covers application and theory of optical spectroscopic phenomena, making connections between theory and emergent areas of research, including biophysics and nanosciences. Since the first optical measurement of a single molecule, over twenty years ago, the field has expanded to include chemical imaging with spatial resolutions that greatly surpass the diffraction limit. These advances have been recognized by a 2014 Nobel Prize. Upon completion of this short course, students will understand the differences in information content of various types of optical imaging signals and acquire the knowledge needed for practical design of a single-molecule spectroscopy or spatially resolved measurement. Topics include: electronic and vibrational spectroscopy, fluorescence, single-molecule spectroscopy, resonance energy transfer and exciton interactions. Applications of nanophotonic materials for increasing the information content of optical measurements will be emphasized. The concepts will be extended to describe recent advances made in improving the temporal and spatial resolution of optical imaging techniques.
- Properties of light
- Light sources
- Properties of LASERS
Basic concepts of quantum mechanics
- Wavefunctions, operators, and expectation values
- Spatial wavefunctions
- Spin wavefunctions
- Time-dependent perturbation theory
- Lifetimes of states and the Uncertainty Principle
- Optical properties of quantum-confined nanoparticles
- Light-matter interactions of plasmonic nanoparticles
- Plasmonic optical signal amplification and transduction
- Fluorescence emission and anisotropy
- Resonance Energy Transfer
- Single-molecule fluorescence spectroscopy
Fluorescence and Nonlinear Optical Microscopy
- Energy-resolving power
- Temporal resolution
The instructor will provide all required reading materials. Many lectures will be derived from relevant literature. Some examples of key papers to be discussed include:
Phys. Rev. B, volume 67, 125304 (2003).
Science, volume 303, pp. 676-678 (2004).
Acc. Chem. Res. Volume 38, 574-582 (2005).
Optional Supplemental Reading:
J. R. Lakowicz, “Principles of Fluorescence Spectroscopy”
William W. Parson. “Modern Optical Spectroscopy With Examples from Biophysics and Biochemistry”