Biomolecules: structure and function
Description of structural and functional properties of biomolecules, focusing on proteins.
By considering common and innovative analytical techniques, different proteins and
metabolic processes are described in relation with diseases by giving an overview in
Program course aa 2014-2015
Introduction of molecular biology, biotechnology, peptides and proteins.
Functional groups in biological molecules. Inter- Intra-molecular bonds. Hydrogen bonds.
Charged molecules. Water solubility. Acidity, basicity, charges at different pHs. pKa. Eq.
Natural, non natural amino acids. Peptide bonds. Synthesis. Character of peptide bond.
Charge and polarity. Proteins. Primary structure. Higher organization levels. Folding and
stability. Inter and intra molecular interactions. Structure activity relationship. Cross-linking.
Structure determination. Structural proteins: collagen, elastin and silk proteins. Muscles
protein and movement. Molecular machines. Globular proteins. Mioglobin and
Hemoglobin: transport, kinetics, cooperative effect, structural modification. Antibodies.
Enzymes. Mechanism of catalysis. Serine proteases. Enzyme kinetic. Inhibition and
inactivation. Enzyme regulation. Hydrolases, lyases, ligases, kinases.
Membrane proteins. Ion transport, ion channel. Passive and active transport.
Analytical applications. Spectrophotometric techniques and light-scattering.
Electrochemical and electrophoretic methods. Chromatography and mass spectrometry.
Thermodynamics in the transformation of high energy compounds. Metabolism of
carbohydrates, lipids, amino acids. Glycolysis. Krebs cycle. Gluconeogenesis. Glycogen.
Metabolism of amino acids and lipids.
David Whitford, Proteins: Structure and Function, Editor: John Wiley & sons, Ltd, ISBN:
Nelson & Cox, Introduzione alla biochimica di Lehninger, Editor: Zanichelli, ISBN:
D. Voet, JG Voet, CW Pratt, Fondamenti di Biochimica, Editor: Zanichelli, ISBN: